Description：Rabbit polyclonal antibody to CRK (Phospho-Y221)Immunogen：KLH-conjugated synthetic phosphopeptide corresponding to residues surrounding Y221 of human CRK protein. The exact sequence is proprietary.Purification：The antibody was purified by immunogen affinity chromatography.Clonality：PolyclonalForm：Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.Dilution：WB (1/500 - 1/1000), IF/IC (1/100 - 1/500)Gene Symbol：CRKAlternative Names：Adapter molecule crk; Proto-oncogene c-Crk; p38 Entrez Gene (Human)： 1398; Entrez Gene (Mouse)： 12928; Entrez Gene (Rat)： 54245; SwissProt (Human)： P46108; SwissProt (Mouse)： Q64010; SwissProt (Rat)： Q63768; Storage/Stability：Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

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  Western blot analysis of CRK (Phospho-Y221) expression in Hela (A), THP1 (B), mouse liver (C), rat liver (D) whole cell lysates. (Predicted band size: 33 kD; Observed band size: 46 kD)
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  Immunofluorescent analysis of CRK (Phospho-Y221) staining in HuvEc cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with Alexa Fluor 647-conjugated secondary antibody (red) in PBS at room temperature in the dark.