Description：Rabbit polyclonal antibody to HMB45Immunogen：KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human HMB45. The exact sequence is proprietary.Purification：The antibody was purified by immunogen affinity chromatography.Clonality：PolyclonalForm：Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.Dilution：WB (1/500 - 1/1000), IH (1/50 - 1/100)Gene Symbol：PMELAlternative Names：D12S53E; PMEL17; SILV; Melanocyte protein PMEL; ME20-M; ME20M; Melanocyte protein Pmel 17; Melanocytes lineage-specific antigen GP100; Melanoma-associated ME20 antigen; P1; P100; Premelanosome protein; Silver locus protein homolog Entrez Gene (Human)： 6490; SwissProt (Human)： P40967; SwissProt (Mouse)： Q60696; Storage/Stability：Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

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  Western blot analysis of HMB45 expression in A375 (A), PC12 (B), AML12 (C), mouse kidney (D), rat ovary (E) whole cell lysates. (Predicted band size: 70 kD; Observed band size: 70; 100 kD)
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  Immunohistochemical analysis of HMB45 staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.