Description:Rabbit polyclonal antibody to GPR173Immunogen:KLH-conjugated synthetic peptide encompassing a sequence within the center region of human GPR173. The exact sequence is proprietary.Purification:The antibody was purified by immunogen affinity chromatography.Clonality:PolyclonalForm:Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.Dilution:WB (1/500 - 1/1000), IF/IC (1/100 - 1/500)Gene Symbol:GPR173Alternative Names:SREB3; Probable G-protein coupled receptor 173; Super conserved receptor expressed in brain 3
Entrez Gene (Human):
54328;
Entrez Gene (Mouse):
70771;
Entrez Gene (Rat):
64021;
SwissProt (Human):
Q9NS66;
SwissProt (Mouse):
Q6PI62;
SwissProt (Rat):
Q9JJH2;
Storage/Stability:Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of GPR173 expression in CT26 (A), C6 (B) whole cell lysates. (Predicted band size: 41 kD; Observed band size: 41 kD)
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Immunofluorescent analysis of GPR173 staining in LOVO cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with Alexa Fluor 647-conjugated secondary antibody (red) in PBS at room temperature in the dark.