Description：Rabbit polyclonal antibody to DNA Polymerase iotaImmunogen：KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human DNA Polymerase iota. The exact sequence is proprietary.Purification：The antibody was purified by immunogen affinity chromatography.Clonality：PolyclonalForm：Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.Dilution：WB (1/500 - 1/1000), IH (1/50 - 1/100)Gene Symbol：POLIAlternative Names：RAD30B; DNA polymerase iota; Eta2; RAD30 homolog B Entrez Gene (Human)： 11201; SwissProt (Human)： Q9UNA4; Storage/Stability：Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

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  Western blot analysis of DNA Polymerase iota expression in A549 (A), U87MG (B), MCF7 (C) whole cell lysates. (Predicted band size: 83 kD; Observed band size: 90 kD)
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  Immunohistochemical analysis of DNA Polymerase iota staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.