Description：Rabbit polyclonal antibody to BRK (Phospho-Y342)Immunogen：KLH-conjugated synthetic phosphopeptide corresponding to residues surrounding Y342 of human BRK protein. The exact sequence is proprietary.Purification：The antibody was purified by immunogen affinity chromatography.Clonality：PolyclonalForm：Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.Dilution：WB (1/500 - 1/1000), IH (1/50 - 1/100)Gene Symbol：PTK6Alternative Names：BRK; Protein-tyrosine kinase 6; Breast tumor kinase; Tyrosine-protein kinase BRK Entrez Gene (Human)： 5753; Entrez Gene (Mouse)： 20459; SwissProt (Human)： Q13882; SwissProt (Mouse)： Q64434; Storage/Stability：Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

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  Western blot analysis of BRK (Phospho-Y342) expression in HEK293T (A), HCT116 (B), NIH3T3 (C) whole cell lysates. (Predicted band size: 51 kD; Observed band size: 51 kD)
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  Immunohistochemical analysis of BRK (Phospho-Y342) staining in human stomach cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.