Description:Rabbit polyclonal antibody to CD45 (Phospho-Y1216)Immunogen:KLH-conjugated synthetic phosphopeptide corresponding to residues surrounding Y1216 of human CD45 protein. The exact sequence is proprietary.Purification:The antibody was purified by immunogen affinity chromatography.Clonality:PolyclonalForm:Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.Dilution:WB (1/500 - 1/1000)Gene Symbol:PTPRCAlternative Names:CD45; Receptor-type tyrosine-protein phosphatase C; Leukocyte common antigen; L-CA; T200; CD45
Entrez Gene (Human):
5788;
Entrez Gene (Rat):
24699;
SwissProt (Human):
P08575;
SwissProt (Mouse):
P06800;
SwissProt (Rat):
P04157;
Storage/Stability:Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of CD45 (Phospho-Y1216) expression in HEK293T (A), MCF7 (B), PC12 (C), AML12 (D) whole cell lysates. (Predicted band size: 147 kD; Observed band size: 180 kD)
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Direct ELISA antibody dose-response curve using Anti-CD45 (Phospho-Y1216) Antibody. Antigen (Phosphopeptide and non-phosphopeptide) concentration is 5 ug/ml. Goat Anti-Rabbit IgG (H&L) - HRP was used as the secondary antibody, and signal was developed by TMB substrate.