Description：Rabbit polyclonal antibody to GPR66Immunogen：KLH-conjugated synthetic peptide encompassing a sequence within the N-term region of human GPR66. The exact sequence is proprietary.Purification：The antibody was purified by immunogen affinity chromatography.Clonality：PolyclonalForm：Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.Dilution：WB (1/500 - 1/1000), IF/IC (1/100 - 1/500)Gene Symbol：NMUR1Alternative Names：GPR66; Neuromedin-U receptor 1; NMU-R1; G-protein coupled receptor 66; G-protein coupled receptor FM-3 Entrez Gene (Human)： 10316; Entrez Gene (Mouse)： 14767; Entrez Gene (Rat)： 65276; SwissProt (Human)： Q9HB89; SwissProt (Mouse)： O55040; SwissProt (Rat)： Q9JJI5; Storage/Stability：Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

• 
  Western blot analysis of GPR66 expression in rat testis (A), mouse testis (B), SGC7901 (C), K562 (D) whole cell lysates. (Predicted band size: 47 kD; Observed band size: 47 kD)
• 
  Immunofluorescent analysis of GPR66 staining in LOVO cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with Alexa Fluor 647-conjugated secondary antibody (red) in PBS at room temperature in the dark.