Description:Rabbit polyclonal antibody to DCLK2Immunogen:KLH-conjugated synthetic peptide encompassing a sequence within the N-term region of human DCLK2. The exact sequence is proprietary.Purification:The antibody was purified by immunogen affinity chromatography.Clonality:PolyclonalForm:Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.Dilution:WB (1/500 - 1/2000), IF/IC (1/50 - 1/100)Gene Symbol:DCLK2Alternative Names:DCAMKL2; DCDC3B; DCK2; Serine/threonine-protein kinase DCLK2; CaMK-like CREB regulatory kinase 2; CL2; CLICK-II; CLICK2; Doublecortin domain-containing protein 3B; Doublecortin-like and CAM kinase-like 2; Doublecortin-like kinase 2
Entrez Gene (Human):
166614;
Entrez Gene (Mouse):
70762;
Entrez Gene (Rat):
310698;
SwissProt (Human):
Q8N568;
SwissProt (Mouse):
Q6PGN3;
SwissProt (Rat):
Q5MPA9;
Storage/Stability:Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of DCLK2 expression in H1792 (A), mouse testis (B), mouse heart (C) whole cell lysates. (Predicted band size: 83 kD; Observed band size: 84 kD)
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Immunofluorescent analysis of DCLK2 staining in HepG2 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).