Description：Rabbit polyclonal antibody to GAP43 (Phospho-S41)Immunogen：KLH-conjugated synthetic phosphopeptide corresponding to residues surrounding S41 of human GAP43 protein. The exact sequence is proprietary.Purification：The antibody was purified by immunogen affinity chromatography.Clonality：PolyclonalForm：Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.Dilution：WB (1/500 - 1/2000), IF/IC (1/50 - 1/100)Gene Symbol：GAP43Alternative Names：Neuromodulin; Axonal membrane protein GAP-43; Growth-associated protein 43; Neural phosphoprotein B-50; pp46 Entrez Gene (Human)： 2596; Entrez Gene (Mouse)： 14432; Entrez Gene (Rat)： 29423; SwissProt (Human)： P17677; SwissProt (Mouse)： P06837; SwissProt (Rat)： P07936; Storage/Stability：Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

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  Western blot analysis of GAP43 (Phospho-S41) expression in HeLa PMA-treated (A), SHSY5Y (B) whole cell lysates. (Predicted band size: 24 kD; Observed band size: 24 kD)
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  Immunofluorescent analysis of GAP43 (Phospho-S41) staining in SHSY5Y cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).