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CPA5371
  • Western blot analysis of SMAD2/3 (Acetyl-K19) expression in zebrafish (A) whole cell lysates. (Predicted band size: 52; 48 kD; Observed band size: 52 kD)
  • Immunohistochemical analysis of SMAD2/3 (Acetyl-K19) staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Product Name:Anti-SMAD2/3 (Acetyl-K19) Antibody
Cat No:CPA5371
Source:Rabbit
Reactivity:H, M, R, B, C, P, Z
Applications:WB, IH
*Application Key:
H - Human, M - Mouse, R - Rat, B - Bovine, C - Chicken, D - Dog, G - Goat, Mk - Monkey, P - Pig, Rb - Rabbit, S - Sheep, Z - Zebrafish
*Species Reactivity Key:
E- ELISA, WB - Western blot, IH - Immunohistochemistry, IF - Immunofluorescence, FC - Flow cytometry, IC - Immunocytochemistry, IP - Immunoprecipitation, ChIP - Chromatin Immunoprecipitation, EMSA - Electrophoretic Mobility Shift Assay, BL - Blocking, SE - Sandwich ELISA, CBE - Cell-based ELISA, RNAi - RNA interference
Size
Price(USD)
200 μl
420
100 μl
260
30 μl
130
50 μl
170
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Description:Rabbit polyclonal antibody to SMAD2/3 (Acetyl-K19)
Immunogen:KLH-conjugated synthetic acetylated peptide corresponding to residues surrounding K19 of human SMAD2/3 protein. The exact sequence is proprietary.
Purification:The antibody was purified by immunogen affinity chromatography.
Clonality:Polyclonal
Form:Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Dilution:WB (1/500 - 1/1000), IH (1/50 - 1/100)
Gene Symbol:SMAD2; SMAD3
Alternative Names:SMAD2; MADH2; MADR2; Mothers against decapentaplegic homolog 2; MAD homolog 2; Mothers against DPP homolog 2; JV18-1; Mad-related protein 2; hMAD-2; SMAD family member 2; SMAD 2; Smad2; hSMAD2; SMAD3; MADH3; Mothers against decapentaplegic homolog 3; MAD homolog 3; Mad3; Mothers against DPP homolog 3; hMAD-3; JV15-2; SMAD family member 3; SMAD 3; Smad3; hSMAD3
Entrez Gene (Human): 4087; 4088;
Entrez Gene (Mouse): 17126; 17127;
Entrez Gene (Rat): 29357; 25631;
SwissProt (Human): Q15796; P84022;
SwissProt (Mouse): Q62432; Q8BUN5;
SwissProt (Rat): O70436; P84025;
Storage/Stability:Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
  • Western blot analysis of SMAD2/3 (Acetyl-K19) expression in zebrafish (A) whole cell lysates. (Predicted band size: 52; 48 kD; Observed band size: 52 kD)
  • Immunohistochemical analysis of SMAD2/3 (Acetyl-K19) staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
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