Description：Rabbit polyclonal antibody to PEA15Immunogen：KLH-conjugated synthetic peptide encompassing a sequence within the center region of human PEA15. The exact sequence is proprietary.Purification：The antibody was purified by immunogen affinity chromatography.Clonality：PolyclonalForm：Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.Dilution：WB (1/500 - 1/2000), IF/IC (1/50 - 1/100)Gene Symbol：PEA15Alternative Names：Astrocytic phosphoprotein PEA-15; 15 kDa phosphoprotein enriched in astrocytes; Phosphoprotein enriched in diabetes; PED Entrez Gene (Human)： 8682; Entrez Gene (Mouse)： 18611; Entrez Gene (Rat)： 364052; SwissProt (Human)： Q15121; SwissProt (Mouse)： Q62048; SwissProt (Rat)： Q5U318; Storage/Stability：Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

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  Western blot analysis of PEA15 expression in HEK293T (A), RAW264.7 (B), H9C2 (C) whole cell lysates. (Predicted band size: 15 kD; Observed band size: 15 kD)
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  Immunofluorescent analysis of PEA15 staining in HEK293T cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).