Description:Rabbit polyclonal antibody to SPTBN1Immunogen:KLH-conjugated synthetic peptide encompassing a sequence within the center region of human SPTBN1. The exact sequence is proprietary.Purification:The antibody was purified by immunogen affinity chromatography.Clonality:PolyclonalForm:Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.Dilution:WB (1/500 - 1/1000), IH (1/100 - 1/200)Gene Symbol:SPTBN1Alternative Names:SPTB2; Spectrin beta chain, non-erythrocytic 1; Beta-II spectrin; Fodrin beta chain; Spectrin, non-erythroid beta chain 1
Entrez Gene (Human):
6711;
Entrez Gene (Mouse):
20742;
SwissProt (Human):
Q01082;
SwissProt (Mouse):
Q62261;
Storage/Stability:Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of SPTBN1 expression in Hela (A), Colo205 (B) whole cell lysates. (Predicted band size: 274 kD; Observed band size: 270 kD)
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Immunohistochemical analysis of SPTBN1 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.