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CPA5165
  • Western blot analysis of SUPT16H expression in H446 (A), mouse lung (B), mouse kidney (C), rat lung (D) whole cell lysates. (Predicted band size: 119 kD; Observed band size: 119 kD)
  • Immunohistochemical analysis of SUPT16H staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Product Name:Anti-SUPT16H Antibody
Cat No:CPA5165
Source:Rabbit
Reactivity:H, M, R
Applications:WB, IH
*Application Key:
H - Human, M - Mouse, R - Rat, B - Bovine, C - Chicken, D - Dog, G - Goat, Mk - Monkey, P - Pig, Rb - Rabbit, S - Sheep, Z - Zebrafish
*Species Reactivity Key:
E- ELISA, WB - Western blot, IH - Immunohistochemistry, IF - Immunofluorescence, FC - Flow cytometry, IC - Immunocytochemistry, IP - Immunoprecipitation, ChIP - Chromatin Immunoprecipitation, EMSA - Electrophoretic Mobility Shift Assay, BL - Blocking, SE - Sandwich ELISA, CBE - Cell-based ELISA, RNAi - RNA interference
Size
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200 μl
350
100 μl
220
30 μl
110
50 μl
150
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Description:Rabbit polyclonal antibody to SUPT16H
Immunogen:KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human SUPT16H. The exact sequence is proprietary.
Purification:The antibody was purified by immunogen affinity chromatography.
Clonality:Polyclonal
Form:Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Dilution:WB (1/500 - 1/1000), IH (1/100 - 1/200)
Gene Symbol:SUPT16H
Alternative Names:FACT140; FACTP140; FACT complex subunit SPT16; Chromatin-specific transcription elongation factor 140 kDa subunit; FACT 140 kDa subunit; FACTp140; Facilitates chromatin transcription complex subunit SPT16; hSPT16
Entrez Gene (Human): 11198;
Entrez Gene (Mouse): 114741;
SwissProt (Human): Q9Y5B9;
SwissProt (Mouse): Q920B9;
Storage/Stability:Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
  • Western blot analysis of SUPT16H expression in H446 (A), mouse lung (B), mouse kidney (C), rat lung (D) whole cell lysates. (Predicted band size: 119 kD; Observed band size: 119 kD)
  • Immunohistochemical analysis of SUPT16H staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
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