Description:Rabbit polyclonal antibody to p47 phox (Phospho-S345)Immunogen:KLH-conjugated synthetic phosphopeptide corresponding to residues surrounding S345 of human p47 phox protein. The exact sequence is proprietary.Purification:The antibody was purified by immunogen affinity chromatography.Clonality:PolyclonalForm:Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.Dilution:WB (1/500 - 1/1000), IH (1/100 - 1/200)Gene Symbol:NCF1Alternative Names:NOXO2; SH3PXD1A; Neutrophil cytosol factor 1; NCF-1; 47 kDa autosomal chronic granulomatous disease protein; 47 kDa neutrophil oxidase factor; NCF-47K; Neutrophil NADPH oxidase factor 1; Nox organizer 2; Nox-organizing protein 2; SH3 and PX domain-containing protein 1A; p47-phox
Entrez Gene (Human):
653361;
Entrez Gene (Mouse):
17969;
SwissProt (Human):
P14598;
SwissProt (Mouse):
Q09014;
Storage/Stability:Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of p47 phox (Phospho-S345) expression in rat lung (A), rat liver (B) whole cell lysates. (Predicted band size: 44 kD; Observed band size: 47 kD)
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Immunohistochemical analysis of p47 phox (Phospho-S345) staining in human lymph node formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.