Description:Rabbit polyclonal antibody to CD276Immunogen:KLH-conjugated synthetic peptide encompassing a sequence within the center region of human CD276. The exact sequence is proprietary.Purification:The antibody was purified by immunogen affinity chromatography.Clonality:PolyclonalForm:Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.Dilution:WB (1/500 - 1/1000), IH (1/50 - 1/100)Gene Symbol:CD276Alternative Names:B7H3; CD276 antigen; 4Ig-B7-H3; B7 homolog 3; B7-H3; Costimulatory molecule; CD276
Entrez Gene (Human):
80381;
Entrez Gene (Mouse):
102657;
Entrez Gene (Rat):
315716;
SwissProt (Human):
Q5ZPR3;
SwissProt (Mouse):
Q8VE98;
SwissProt (Rat):
Q7TPB4;
Storage/Stability:Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of CD276 expression in MCF7 (A), HEK293T (B) whole cell lysates. (Predicted band size: 57 kD; Observed band size: 57 kD)
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Immunohistochemical analysis of CD276 staining in human liver formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.