Description:Rabbit polyclonal antibody to iNOSImmunogen:KLH-conjugated synthetic peptide encompassing a sequence within the N-term region of human iNOS. The exact sequence is proprietary.Purification:The antibody was purified by immunogen affinity chromatography.Clonality:PolyclonalForm:Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.Dilution:WB (1/500 - 1/1000), IH (1/100 - 1/200)Gene Symbol:NOS2Alternative Names:NOS2A; Nitric oxide synthase, inducible; Hepatocyte NOS; HEP-NOS; Inducible NO synthase; Inducible NOS; iNOS; NOS type II; Peptidyl-cysteine S-nitrosylase NOS2
Entrez Gene (Human):
4843;
Entrez Gene (Mouse):
18126;
Entrez Gene (Rat):
24599;
SwissProt (Human):
P35228;
SwissProt (Mouse):
P29477;
SwissProt (Rat):
Q06518;
Storage/Stability:Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of iNOS expression in BV2 (A), LO2 (B), HepG2 (C) whole cell lysates. (Predicted band size: 131 kD; Observed band size: 131 kD)
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Immunohistochemical analysis of iNOS staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.