Description：Rabbit polyclonal antibody to SPHK2 (Phospho-T614)Immunogen：KLH-conjugated synthetic phosphopeptide corresponding to residues surrounding T614 of human SPHK2 protein. The exact sequence is proprietary.Purification：The antibody was purified by immunogen affinity chromatography.Clonality：PolyclonalForm：Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.Dilution：WB (1/500 - 1/1000), IH (1/100 - 1/200)Gene Symbol：SPHK2Alternative Names：Sphingosine kinase 2; SK 2; SPK 2 Entrez Gene (Human)： 56848; Entrez Gene (Mouse)： 56632; SwissProt (Human)： Q9NRA0; SwissProt (Mouse)： Q9JIA7; Storage/Stability：Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

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  Western blot analysis of SPHK2 (Phospho-T614) expression in HEK293T (A), HepG2 (B) whole cell lysates. (Predicted band size: 69 kD; Observed band size: 70 kD)
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  Immunohistochemical analysis of SPHK2 (Phospho-T614) staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.