Description:Rabbit polyclonal antibody to NMDAR2B (Phospho-Y1474)Immunogen:KLH-conjugated synthetic phosphopeptide corresponding to residues surrounding Y1474 of human NMDAR2B protein. The exact sequence is proprietary.Purification:The antibody was purified by immunogen affinity chromatography.Clonality:PolyclonalForm:Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.Dilution:WB (1/500 - 1/1000), IH (1/50 - 1/100)Gene Symbol:GRIN2BAlternative Names:NMDAR2B; Glutamate receptor ionotropic, NMDA 2B; GluN2B; Glutamate [NMDA] receptor subunit epsilon-2; N-Methyl-D-aspartate receptor subtype 2B; NMDAR2B; NR2B; N-methyl-D-aspartate receptor subunit 3; NR3; hNR3
Entrez Gene (Human):
2904;
Entrez Gene (Mouse):
14812;
Entrez Gene (Rat):
24410;
SwissProt (Human):
Q13224;
SwissProt (Mouse):
Q01097;
SwissProt (Rat):
Q00960;
Storage/Stability:Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of NMDAR2B (Phospho-Y1474) expression in HEK293T UV-treated (A), mouse brain (B), rat brain (C) whole cell lysates. (Predicted band size: 166 kD; Observed band size: 190 kD)
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Immunohistochemical analysis of NMDAR2B (Phospho-Y1474) staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.