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CPA4496
  • Western blot analysis of PTEN expression in MCF7 (A), HepG2 (B) whole cell lysates. (Predicted band size: 47 kD; Observed band size: 54 kD)
  • Immunohistochemical analysis of PTEN staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Product Name:Anti-PTEN Antibody
Cat No:CPA4496
Source:Rabbit
Reactivity:H, M, B, D, Mk, P, Z
Applications:WB, IH
*Application Key:
H - Human, M - Mouse, R - Rat, B - Bovine, C - Chicken, D - Dog, G - Goat, Mk - Monkey, P - Pig, Rb - Rabbit, S - Sheep, Z - Zebrafish
*Species Reactivity Key:
E- ELISA, WB - Western blot, IH - Immunohistochemistry, IF - Immunofluorescence, FC - Flow cytometry, IC - Immunocytochemistry, IP - Immunoprecipitation, ChIP - Chromatin Immunoprecipitation, EMSA - Electrophoretic Mobility Shift Assay, BL - Blocking, SE - Sandwich ELISA, CBE - Cell-based ELISA, RNAi - RNA interference
Size
Price(USD)
200 μl
350
100 μl
220
30 μl
110
50 μl
150
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Description:Rabbit polyclonal antibody to PTEN
Immunogen:KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human PTEN. The exact sequence is proprietary.
Purification:The antibody was purified by immunogen affinity chromatography.
Clonality:Polyclonal
Form:Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Dilution:WB (1/500 - 1/1000), IH (1/100 - 1/200)
Gene Symbol:PTEN
Alternative Names:MMAC1; TEP1; Phosphatidylinositol 3,4,5-trisphosphate 3-phosphatase and dual-specificity protein phosphatase PTEN; Mutated in multiple advanced cancers 1; Phosphatase and tensin homolog
Entrez Gene (Human): 5728;
Entrez Gene (Mouse): 19211;
SwissProt (Human): P60484;
SwissProt (Mouse): O08586;
Storage/Stability:Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
  • Western blot analysis of PTEN expression in MCF7 (A), HepG2 (B) whole cell lysates. (Predicted band size: 47 kD; Observed band size: 54 kD)
  • Immunohistochemical analysis of PTEN staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Simultaneous inactivation of Par-4 and PTEN in vivo leads to synergistic NF-kappaB activation and invasive prostate carcinoma
Journal Proc Natl Acad Sci U S A
IF
Application IHC-P
Reactivity Mouse
PMID 19470463
Genomic Deletion of PTEN Is Associated with Tumor Progression and Early PSA Recurrence in ERG Fusion-Positive and Fusion-Negative Prostate Cancer
Journal Am J Pathol
IF 6
Application IHC-P
Reactivity Human
PMID 22705054
The oncogenic microRNA OncomiR-21 overexpressed during Marek's disease lymphomagenesis is transactivated by the viral oncoprotein Meq
Journal J Virol
IF 5.4
Application
Reactivity
PMID 23055556
A novel PTEN gene promoter mutation and untypical Cowden syndrome
Journal Chin J Cancer Res
IF 5.1
Application
Reactivity
PMID 23825907
SPOP suppresses tumorigenesis by regulating hedgehog/Gli2 signaling pathway in gastric cancer
Journal J Exp Clin Cancer Res
IF 11.3
Application
Reactivity Human
PMID 25204354
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