Description:Rabbit polyclonal antibody to MEF2AImmunogen:KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human MEF2A. The exact sequence is proprietary.Purification:The antibody was purified by immunogen affinity chromatography.Clonality:PolyclonalForm:Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.Dilution:WB (1/500 - 1/1000), IF/IC (1/50 - 1/200)Gene Symbol:MEF2AAlternative Names:MEF2; Myocyte-specific enhancer factor 2A; Serum response factor-like protein 1
Entrez Gene (Human):
4205;
Entrez Gene (Mouse):
17258;
Entrez Gene (Rat):
309957;
SwissProt (Human):
Q02078;
SwissProt (Mouse):
Q60929;
SwissProt (Rat):
Q2MJT0;
Storage/Stability:Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of MEF2A expression in mouse heart (A), rat heart (B) whole cell lysates. (Predicted band size: 54 kD; Observed band size: 55 kD)
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Immunofluorescent analysis of MEF2A staining in Hela cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.
MHCI requires MEF2 transcription factors to negatively regulate synapse density during development and in disease