Description:Rabbit polyclonal antibody to GSK3 beta (Phospho-S9)Immunogen:KLH-conjugated synthetic phosphopeptide corresponding to residues surrounding S9 of human GSK3 beta protein. The exact sequence is proprietary.Purification:The antibody was purified by immunogen affinity chromatography.Clonality:PolyclonalForm:Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.Dilution:WB (1/500 - 1/1000), IH (1/100 - 1/200)Gene Symbol:GSK3BAlternative Names:Glycogen synthase kinase-3 beta; GSK-3 beta; Serine/threonine-protein kinase GSK3B
Entrez Gene (Human):
2932;
SwissProt (Human):
P49841;
Storage/Stability:Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of GSK3 beta (Phospho-S9) expression in Jurkat (A), MCF7 (B), PC3 (C) whole cell lysates. (Predicted band size: 46 kD; Observed band size: 46 kD)
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Immunohistochemical analysis of GSK3 beta (Phospho-S9) staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.