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CPA4290
  • Western blot analysis of CARK expression in HEK293T (A), Raw264.7 (B), H9C2 (C) whole cell lysates. (Predicted band size: 92 kD; Observed band size: 92 kD)
  • Immunohistochemical analysis of CARK staining in human heart formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Product Name:Anti-CARK Antibody
Cat No:CPA4290
Source:Rabbit
Reactivity:H, M, R, B
Applications:WB, IH
*Application Key:
H - Human, M - Mouse, R - Rat, B - Bovine, C - Chicken, D - Dog, G - Goat, Mk - Monkey, P - Pig, Rb - Rabbit, S - Sheep, Z - Zebrafish
*Species Reactivity Key:
E- ELISA, WB - Western blot, IH - Immunohistochemistry, IF - Immunofluorescence, FC - Flow cytometry, IC - Immunocytochemistry, IP - Immunoprecipitation, ChIP - Chromatin Immunoprecipitation, EMSA - Electrophoretic Mobility Shift Assay, BL - Blocking, SE - Sandwich ELISA, CBE - Cell-based ELISA, RNAi - RNA interference
Size
Price(USD)
200 μl
350
100 μl
220
30 μl
110
50 μl
150
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Description:Rabbit polyclonal antibody to CARK
Immunogen:KLH-conjugated synthetic peptide encompassing a sequence within the center region of human CARK. The exact sequence is proprietary.
Purification:The antibody was purified by immunogen affinity chromatography.
Clonality:Polyclonal
Form:Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Dilution:WB (1/500 - 1/1000), IH (1/100 - 1/200)
Gene Symbol:TNNI3K
Alternative Names:CARK; Serine/threonine-protein kinase TNNI3K; Cardiac ankyrin repeat kinase; Cardiac troponin I-interacting kinase; TNNI3-interacting kinase
Entrez Gene (Human): 100526835; 51086;
Entrez Gene (Mouse): 435766;
Entrez Gene (Rat): 295531;
SwissProt (Human): Q59H18;
SwissProt (Mouse): Q5GIG6;
SwissProt (Rat): Q7TQP6;
Storage/Stability:Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
  • Western blot analysis of CARK expression in HEK293T (A), Raw264.7 (B), H9C2 (C) whole cell lysates. (Predicted band size: 92 kD; Observed band size: 92 kD)
  • Immunohistochemical analysis of CARK staining in human heart formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
TNNI3K mutation in familial syndrome of conduction system disease, atrial tachyarrhythmia and dilated cardiomyopathy
Journal Hum Mol Genet
IF 3.5
Application WB
Reactivity Mouse
PMID 24925317
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