Welcome to Cohesion Biosciences
Home> Products > Primary Antibody
CPA4152
  • Western blot analysis of PIAS4 expression in HepG2 (A), HeLa (B) whole cell lysates. (Predicted band size: 56 kD; Observed band size: 75 kD)
  • Immunohistochemical analysis of PIAS4 staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Product Name:Anti-PIAS4 Antibody
Cat No:CPA4152
Source:Rabbit
Reactivity:H, B
Applications:WB, IH
*Application Key:
H - Human, M - Mouse, R - Rat, B - Bovine, C - Chicken, D - Dog, G - Goat, Mk - Monkey, P - Pig, Rb - Rabbit, S - Sheep, Z - Zebrafish
*Species Reactivity Key:
E- ELISA, WB - Western blot, IH - Immunohistochemistry, IF - Immunofluorescence, FC - Flow cytometry, IC - Immunocytochemistry, IP - Immunoprecipitation, ChIP - Chromatin Immunoprecipitation, EMSA - Electrophoretic Mobility Shift Assay, BL - Blocking, SE - Sandwich ELISA, CBE - Cell-based ELISA, RNAi - RNA interference
Size
Price(USD)
200 μl
350
100 μl
220
30 μl
110
50 μl
150
Add to cart Ship in 3 days My orders
Description:Rabbit polyclonal antibody to PIAS4
Immunogen:KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human PIAS4. The exact sequence is proprietary.
Purification:The antibody was purified by immunogen affinity chromatography.
Clonality:Polyclonal
Form:Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Dilution:WB (1/500 - 1/1000), IH (1/100 - 1/200)
Gene Symbol:PIAS4
Alternative Names:PIASG; E3 SUMO-protein ligase PIAS4; PIASy; Protein inhibitor of activated STAT protein 4; Protein inhibitor of activated STAT protein gamma; PIAS-gamma
Entrez Gene (Human): 51588;
SwissProt (Human): Q8N2W9;
Storage/Stability:Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
  • Western blot analysis of PIAS4 expression in HepG2 (A), HeLa (B) whole cell lysates. (Predicted band size: 56 kD; Observed band size: 75 kD)
  • Immunohistochemical analysis of PIAS4 staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Caveolin-3 undergoes SUMOylation by the SUMO E3 ligase PIASy: sumoylation affects G-protein-coupled receptor desensitization
Journal J Biol Chem
IF 4.8
Application WB
Reactivity Human
PMID 21362625
PKC{zeta} mediates disturbed flow-induced endothelial apoptosis via p53 SUMOylation
Journal J Cell Biol
IF 7.8
Application
Reactivity
PMID 21624955
COHESION BIOSCIENCES LIMITED
Copyright © 2022 Cohesion Biosciences. All Rights Reserved