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CPA4126
  • Western blot analysis of NOTCH1 expression in Myla2059 (A) whole cell lysates. (Predicted band size: 272 kD; Observed band size: 120 kD)
  • Immunohistochemical analysis of NOTCH1 staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
  • Immunofluorescent analysis of NOTCH1 staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
Product Name:Anti-NOTCH1 Antibody
Cat No:CPA4126
Source:Rabbit
Reactivity:H, M
Applications:WB, IH, IF/IC
*Application Key:
H - Human, M - Mouse, R - Rat, B - Bovine, C - Chicken, D - Dog, G - Goat, Mk - Monkey, P - Pig, Rb - Rabbit, S - Sheep, Z - Zebrafish
*Species Reactivity Key:
E- ELISA, WB - Western blot, IH - Immunohistochemistry, IF - Immunofluorescence, FC - Flow cytometry, IC - Immunocytochemistry, IP - Immunoprecipitation, ChIP - Chromatin Immunoprecipitation, EMSA - Electrophoretic Mobility Shift Assay, BL - Blocking, SE - Sandwich ELISA, CBE - Cell-based ELISA, RNAi - RNA interference
Size
Price(USD)
200 μl
350
100 μl
220
30 μl
110
50 μl
150
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Description:Rabbit polyclonal antibody to NOTCH1
Immunogen:KLH-conjugated synthetic peptide encompassing a sequence within the center region of human NOTCH1. The exact sequence is proprietary.
Purification:The antibody was purified by immunogen affinity chromatography.
Clonality:Polyclonal
Form:Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Dilution:WB (1/500 - 1/1000), IH (1/100 - 1/200), IF/IC (1/100 - 1/500)
Gene Symbol:NOTCH1
Alternative Names:TAN1; Neurogenic locus notch homolog protein 1; Notch 1; hN1; Translocation-associated notch protein TAN-1
Entrez Gene (Human): 4851;
Entrez Gene (Mouse): 18128;
SwissProt (Human): P46531;
SwissProt (Mouse): Q01705;
Storage/Stability:Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
  • Western blot analysis of NOTCH1 expression in Myla2059 (A) whole cell lysates. (Predicted band size: 272 kD; Observed band size: 120 kD)
  • Immunohistochemical analysis of NOTCH1 staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
  • Immunofluorescent analysis of NOTCH1 staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
Preventive effect of Notch signaling inhibition by a gamma-secretase inhibitor on peritoneal dialysis fluid-induced peritoneal fibrosis in rats
Journal Am J Pathol
IF 6
Application WB, IHC-P
Reactivity Rat
PMID 20056840
Notch activation differentially regulates renal progenitors proliferation and differentiation toward the podocyte lineage in glomerular disorders
Journal Stem Cells
IF 5.2
Application ICC/IF
Reactivity Human
PMID 20680961
Inhibition of Fibroblast Growth by Notch1 Signaling Is Mediated by Induction of Wnt11-Dependent WISP-1
Journal PLoS One
IF 3.7
Application WB
Reactivity Human
PMID 22715413
Gene expression and functional annotation of the human ciliary body epithelia
Journal PLoS One
IF 3.7
Application IHC-FoFr
Reactivity Human
PMID 23028713
Downregulation of microRNA-30 facilitates podocyte injury and is prevented by glucocorticoids
Journal J Am Soc Nephrol
IF 13.6
Application WB
Reactivity Mouse
PMID 24029422
The jagged-2/notch-1/hes-1 pathway is involved in intestinal epithelium regeneration after intestinal ischemia-reperfusion injury
Journal PLoS One
IF 3.7
Application WB, IHC-Fr
Reactivity Rat
PMID 24098462
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