Description:Rabbit polyclonal antibody to p40 phoxImmunogen:KLH-conjugated synthetic peptide encompassing a sequence within the center region of human p40 phox. The exact sequence is proprietary.Purification:The antibody was purified by immunogen affinity chromatography.Clonality:PolyclonalForm:Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.Dilution:WB (1/500 - 1/1000), IH (1/100 - 1/200)Gene Symbol:NCF4Alternative Names:SH3PXD4; Neutrophil cytosol factor 4; NCF-4; Neutrophil NADPH oxidase factor 4; SH3 and PX domain-containing protein 4; p40-phox; p40phox
Entrez Gene (Human):
4689;
Entrez Gene (Mouse):
17972;
SwissProt (Human):
Q15080;
SwissProt (Mouse):
P97369;
Storage/Stability:Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of p40 phox expression in MCF7 (A) whole cell lysates. (Predicted band size: 39 kD; Observed band size: 40 kD)
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Immunohistochemical analysis of p40 phox staining in human tonsil formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.