Description:Rabbit polyclonal antibody to MSH3Immunogen:KLH-conjugated synthetic peptide encompassing a sequence within the N-term region of human MSH3. The exact sequence is proprietary.Purification:The antibody was purified by immunogen affinity chromatography.Clonality:PolyclonalForm:Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.Dilution:WB (1/500 - 1/1000), IH (1/100 - 1/200)Gene Symbol:MSH3Alternative Names:DUC1; DUG; DNA mismatch repair protein Msh3; hMSH3; Divergent upstream protein; DUP; Mismatch repair protein 1; MRP1
Entrez Gene (Human):
4437;
SwissProt (Human):
P20585;
Storage/Stability:Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of MSH3 expression in A549 (A), MCF7 (B) whole cell lysates. (Predicted band size: 127 kD; Observed band size: 127 kD)
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Immunohistochemical analysis of MSH3 staining in human colon cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Mismatch repair and nucleotide excision repair proteins cooperate in the recognition of DNA interstrand crosslinks