Welcome to Cohesion Biosciences
Home> Products > Primary Antibody
CPA4081
  • Western blot analysis of GW expression in Hela (A), MCF7 (B), AML12 (C) whole cell lysates. (Predicted band size: 97 kD; Observed band size: 93 kD)
  • Immunohistochemical analysis of GW staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Product Name:Anti-GW Antibody
Cat No:CPA4081
Source:Rabbit
Reactivity:H, M, B, C, D
Applications:WB, IH
*Application Key:
H - Human, M - Mouse, R - Rat, B - Bovine, C - Chicken, D - Dog, G - Goat, Mk - Monkey, P - Pig, Rb - Rabbit, S - Sheep, Z - Zebrafish
*Species Reactivity Key:
E- ELISA, WB - Western blot, IH - Immunohistochemistry, IF - Immunofluorescence, FC - Flow cytometry, IC - Immunocytochemistry, IP - Immunoprecipitation, ChIP - Chromatin Immunoprecipitation, EMSA - Electrophoretic Mobility Shift Assay, BL - Blocking, SE - Sandwich ELISA, CBE - Cell-based ELISA, RNAi - RNA interference
Size
Price(USD)
200 μl
350
100 μl
220
30 μl
110
50 μl
150
Add to cart Ship in 3 days My orders
Description:Rabbit polyclonal antibody to GW
Immunogen:KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human GW. The exact sequence is proprietary.
Purification:The antibody was purified by immunogen affinity chromatography.
Clonality:Polyclonal
Form:Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Dilution:WB (1/500 - 1/1000), IH (1/100 - 1/200)
Gene Symbol:MASTL
Alternative Names:GW; GWL; THC2; Serine/threonine-protein kinase greatwall; GW; GWL; hGWL; Microtubule-associated serine/threonine-protein kinase-like; MAST-L
Entrez Gene (Human): 84930;
Entrez Gene (Mouse): 67121;
SwissProt (Human): Q96GX5;
SwissProt (Mouse): Q8C0P0;
Storage/Stability:Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
  • Western blot analysis of GW expression in Hela (A), MCF7 (B), AML12 (C) whole cell lysates. (Predicted band size: 97 kD; Observed band size: 93 kD)
  • Immunohistochemical analysis of GW staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
MASTL is the human orthologue of Greatwall kinase that facilitates mitotic entry, anaphase and cytokinesis
Journal Cell Cycle
IF 4.3
Application WB
Reactivity Human
PMID 20818157
Targeting Mitotic Exit Leads to Tumor Regression In Vivo: Modulation by Cdk1, Mastl, and the PP2A/B55a,d Phosphatase
Journal Cancer Cell
IF 50.3
Application WB
Reactivity Mouse
PMID 21156286
COHESION BIOSCIENCES LIMITED
Copyright © 2022 Cohesion Biosciences. All Rights Reserved