Description:Rabbit polyclonal antibody to GWImmunogen:KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human GW. The exact sequence is proprietary.Purification:The antibody was purified by immunogen affinity chromatography.Clonality:PolyclonalForm:Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.Dilution:WB (1/500 - 1/1000), IH (1/100 - 1/200)Gene Symbol:MASTLAlternative Names:GW; GWL; THC2; Serine/threonine-protein kinase greatwall; GW; GWL; hGWL; Microtubule-associated serine/threonine-protein kinase-like; MAST-L
Entrez Gene (Human):
84930;
Entrez Gene (Mouse):
67121;
SwissProt (Human):
Q96GX5;
SwissProt (Mouse):
Q8C0P0;
Storage/Stability:Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of GW expression in Hela (A), MCF7 (B), AML12 (C) whole cell lysates. (Predicted band size: 97 kD; Observed band size: 93 kD)
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Immunohistochemical analysis of GW staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
MASTL is the human orthologue of Greatwall kinase that facilitates mitotic entry, anaphase and cytokinesis
Targeting Mitotic Exit Leads to Tumor Regression In Vivo: Modulation by Cdk1, Mastl, and the PP2A/B55a,d Phosphatase