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CPA3949
  • Western blot analysis of GABBR1 expression in A375 (A) whole cell lysates. (Predicted band size: 108 kD; Observed band size: 130; 95 kD)
  • Immunofluorescent analysis of GABBR1 staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
Product Name:Anti-GABBR1 Antibody
Cat No:CPA3949
Source:Rabbit
Reactivity:H, R, B, Mk
Applications:WB, IF/IC
*Application Key:
H - Human, M - Mouse, R - Rat, B - Bovine, C - Chicken, D - Dog, G - Goat, Mk - Monkey, P - Pig, Rb - Rabbit, S - Sheep, Z - Zebrafish
*Species Reactivity Key:
E- ELISA, WB - Western blot, IH - Immunohistochemistry, IF - Immunofluorescence, FC - Flow cytometry, IC - Immunocytochemistry, IP - Immunoprecipitation, ChIP - Chromatin Immunoprecipitation, EMSA - Electrophoretic Mobility Shift Assay, BL - Blocking, SE - Sandwich ELISA, CBE - Cell-based ELISA, RNAi - RNA interference
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Description:Rabbit polyclonal antibody to GABBR1
Immunogen:KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human GABBR1. The exact sequence is proprietary.
Purification:The antibody was purified by immunogen affinity chromatography.
Clonality:Polyclonal
Form:Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Dilution:WB (1/500 - 1/1000), IF/IC (1/100 - 1/500)
Gene Symbol:GABBR1
Alternative Names:GPRC3A; Gamma-aminobutyric acid type B receptor subunit 1; GABA-B receptor 1; GABA-B-R1; GABA-BR1; GABABR1; Gb1
Entrez Gene (Human): 2550;
Entrez Gene (Mouse): 54393;
Entrez Gene (Rat): 81657;
SwissProt (Human): Q9UBS5;
SwissProt (Mouse): Q9WV18;
SwissProt (Rat): Q9Z0U4;
Storage/Stability:Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
  • Western blot analysis of GABBR1 expression in A375 (A) whole cell lysates. (Predicted band size: 108 kD; Observed band size: 130; 95 kD)
  • Immunofluorescent analysis of GABBR1 staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
Aminobutyric Acid Type B (GABAB) Receptor Expression Is Needed for Inhibition of N-type (Cav2.2) Calcium Channels by Analgesic a-Conotoxins
Journal J Biol Chem
IF 4.8
Application ICC/IF
Reactivity
PMID 22613715
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