Description：Rabbit polyclonal antibody to Frizzled 1/2/7Immunogen：KLH-conjugated synthetic peptide encompassing a sequence within the N-term region of human Frizzled 1/2/7. The exact sequence is proprietary.Purification：The antibody was purified by immunogen affinity chromatography.Clonality：PolyclonalForm：Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.Dilution：WB (1/500 - 1/1000), IF/IC (1/50 - 1/200)Gene Symbol：FZD1; FZD2; FZD7Alternative Names：Frizzled-7; Fz-7; hFz7; FzE3 Entrez Gene (Human)： 8324; Entrez Gene (Mouse)： 14369; SwissProt (Human)： Q9UP38; Q14332; O75084; SwissProt (Mouse)： Q61090; Storage/Stability：Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

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  Western blot analysis of Frizzled 1/2/7 expression in Hela (A), mouse heart (B), rat heart (C), rat kidney (D) whole cell lysates. (Predicted band size: 71; 63 kD; Observed band size: 64; 71 kD)
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  Immunofluorescent analysis of Frizzled 1/2/7 staining in MCF7 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a AF594-conjugated secondary antibody (red) in PBS at room temperature in the dark.