Description：Rabbit polyclonal antibody to CHK2 (Phospho-T383)Immunogen：KLH-conjugated synthetic phosphopeptide corresponding to residues surrounding T383 of human CHK2 protein. The exact sequence is proprietary.Purification：The antibody was purified by immunogen affinity chromatography.Clonality：PolyclonalForm：Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.Dilution：WB (1/500 - 1/1000), IF/IC (1/100 - 1/500)Gene Symbol：CHEK2Alternative Names：CDS1; CHK2; RAD53; Serine/threonine-protein kinase Chk2; CHK2 checkpoint homolog; Cds1 homolog; Hucds1; hCds1; Checkpoint kinase 2 Entrez Gene (Human)： 11200; Entrez Gene (Mouse)： 50883; SwissProt (Human)： O96017; SwissProt (Mouse)： Q9Z265; Storage/Stability：Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

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  Western blot analysis of CHK2 (Phospho-T383) expression in HEK293T (A), HEK293T-UV-30min (B) whole cell lysates. (Predicted band size: 60 kD; Observed band size: 62 kD)
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  Immunofluorescent analysis of CHK2 (Phospho-T383) staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).