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CPA3781
  • Western blot analysis of ATF5 expression in HEK293T (A), A549 (B), H1688 (C), mouse heart (D), mouse kidney (E), rat heart (F), rat kidney (G) whole cell lysates. (Predicted band size: 30 kD; Observed band size: 38; 30 kD)
  • Immunohistochemical analysis of ATF5 staining in human prostate cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Product Name:Anti-ATF5 Antibody
Cat No:CPA3781
Source:Rabbit
Reactivity:H, M, R
Applications:WB, IH
*Application Key:
H - Human, M - Mouse, R - Rat, B - Bovine, C - Chicken, D - Dog, G - Goat, Mk - Monkey, P - Pig, Rb - Rabbit, S - Sheep, Z - Zebrafish
*Species Reactivity Key:
E- ELISA, WB - Western blot, IH - Immunohistochemistry, IF - Immunofluorescence, FC - Flow cytometry, IC - Immunocytochemistry, IP - Immunoprecipitation, ChIP - Chromatin Immunoprecipitation, EMSA - Electrophoretic Mobility Shift Assay, BL - Blocking, SE - Sandwich ELISA, CBE - Cell-based ELISA, RNAi - RNA interference
Size
Price(USD)
200 μl
350
100 μl
220
30 μl
110
50 μl
150
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Description:Rabbit polyclonal antibody to ATF5
Immunogen:KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human ATF5. The exact sequence is proprietary.
Purification:The antibody was purified by immunogen affinity chromatography.
Clonality:Polyclonal
Form:Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Dilution:WB (1/500 - 1/1000), IH (1/100 - 1/200)
Gene Symbol:ATF5
Alternative Names:ATFX; Cyclic AMP-dependent transcription factor ATF-5; cAMP-dependent transcription factor ATF-5; Activating transcription factor 5; Transcription factor ATFx
Entrez Gene (Human): 22809;
Entrez Gene (Mouse): 107503;
Entrez Gene (Rat): 282840;
SwissProt (Human): Q9Y2D1;
SwissProt (Mouse): O70191;
SwissProt (Rat): Q6P788;
Storage/Stability:Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
  • Western blot analysis of ATF5 expression in HEK293T (A), A549 (B), H1688 (C), mouse heart (D), mouse kidney (E), rat heart (F), rat kidney (G) whole cell lysates. (Predicted band size: 30 kD; Observed band size: 38; 30 kD)
  • Immunohistochemical analysis of ATF5 staining in human prostate cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
p300-dependent acetylation of activating transcription factor 5 enhances C/EBPß transactivation of C/EBPa during 3T3-L1 differentiation
Journal Mol Cell Biol
IF 5.3
Application
Reactivity
PMID 24216764
A High Concentrate Diet Inhibits Forkhead Box Protein A2 Expression, and Induces Oxidative Stress, Mitochondrial Dysfunction and Mitochondrial Unfolded Protein Response in the Liver of Dairy Cows
Journal Microb Pathog
IF 3.8
Application WB
Reactivity Bovine
PMID 38341108
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