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CPA3746
  • Western blot analysis of ACC alpha (Phospho-S80) expression in HEK293T (A) whole cell lysates. (Predicted band size: 265 kD; Observed band size: 280 kD)
  • Immunohistochemical analysis of ACC alpha (Phospho-S80) staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
  • Direct ELISA antibody dose-response curve using Anti-ACC alpha (Phospho-S80) Antibody. Antigen (Phosphopeptide and non-phosphopeptide) concentration is 5 ug/ml. Goat Anti-Rabbit IgG (H&L) - HRP was used as the secondary antibody, and signal was developed by TMB substrate.
Product Name:Anti-ACC alpha (Phospho-S80) Antibody
Cat No:CPA3746
Source:Rabbit
Reactivity:H, M, R, B, P, S
Applications:WB, IH
*Application Key:
H - Human, M - Mouse, R - Rat, B - Bovine, C - Chicken, D - Dog, G - Goat, Mk - Monkey, P - Pig, Rb - Rabbit, S - Sheep, Z - Zebrafish
*Species Reactivity Key:
E- ELISA, WB - Western blot, IH - Immunohistochemistry, IF - Immunofluorescence, FC - Flow cytometry, IC - Immunocytochemistry, IP - Immunoprecipitation, ChIP - Chromatin Immunoprecipitation, EMSA - Electrophoretic Mobility Shift Assay, BL - Blocking, SE - Sandwich ELISA, CBE - Cell-based ELISA, RNAi - RNA interference
Size
Price(USD)
200 μl
420
100 μl
260
30 μl
130
50 μl
170
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Description:Rabbit polyclonal antibody to ACC alpha (Phospho-S80)
Immunogen:KLH-conjugated synthetic phosphopeptide corresponding to residues surrounding S80 of human ACC alpha protein. The exact sequence is proprietary.
Purification:The antibody was purified by immunogen affinity chromatography.
Clonality:Polyclonal
Form:Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Dilution:WB (1/500 - 1/1000), IH (1/100 - 1/200)
Gene Symbol:ACACA
Alternative Names:ACAC; ACC1; ACCA; Acetyl-CoA carboxylase 1; ACC1; ACC-alpha
Entrez Gene (Human): 31;
Entrez Gene (Mouse): 107476;
Entrez Gene (Rat): 60581;
SwissProt (Human): Q13085;
SwissProt (Mouse): Q5SWU9;
SwissProt (Rat): P11497;
Storage/Stability:Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
  • Western blot analysis of ACC alpha (Phospho-S80) expression in HEK293T (A) whole cell lysates. (Predicted band size: 265 kD; Observed band size: 280 kD)
  • Immunohistochemical analysis of ACC alpha (Phospho-S80) staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
  • Direct ELISA antibody dose-response curve using Anti-ACC alpha (Phospho-S80) Antibody. Antigen (Phosphopeptide and non-phosphopeptide) concentration is 5 ug/ml. Goat Anti-Rabbit IgG (H&L) - HRP was used as the secondary antibody, and signal was developed by TMB substrate.
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