Description：Rabbit polyclonal antibody to YKL-40Immunogen：KLH-conjugated synthetic peptide encompassing a sequence within the center region of human YKL-40. The exact sequence is proprietary.Purification：The antibody was purified by immunogen affinity chromatography.Clonality：PolyclonalForm：Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.Dilution：WB (1/500 - 1/1000), IF/IC (1/50 - 1/200)Gene Symbol：CHI3L1Alternative Names：Chitinase-3-like protein 1; 39 kDa synovial protein; Cartilage glycoprotein 39; CGP-39; GP-39; hCGP-39; YKL-40 Entrez Gene (Human)： 1116; Entrez Gene (Mouse)： 12654; Entrez Gene (Rat)： 89824; SwissProt (Human)： P36222; SwissProt (Mouse)： Q61362; SwissProt (Rat)： Q9WTV1; Storage/Stability：Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

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  Western blot analysis of YKL-40 expression in mouse liver (A), rat liver (B) whole cell lysates. (Predicted band size: 42 kD; Observed band size: 42 kD)
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  Immunofluorescent analysis of YKL-40 staining in SGC7901 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark. Phalloidin - AF594 was used to stain Actin filaments (red). DAPI was used to stain the cell nuclei (blue).