Description:Rabbit polyclonal antibody to LPHN1Immunogen:KLH-conjugated synthetic peptide encompassing a sequence within the center region of human LPHN1. The exact sequence is proprietary.Purification:The antibody was purified by immunogen affinity chromatography.Clonality:PolyclonalForm:Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.Dilution:WB (1/500 - 1/1000), IF/IC (1/50 - 1/200)Gene Symbol:LPHN1Alternative Names:KIAA0821; LEC2; Latrophilin-1; Calcium-independent alpha-latrotoxin receptor 1; CIRL-1; Lectomedin-2
Entrez Gene (Human):
22859;
Entrez Gene (Mouse):
330814;
Entrez Gene (Rat):
65096;
SwissProt (Human):
O94910;
SwissProt (Mouse):
Q80TR1;
SwissProt (Rat):
O88917;
Storage/Stability:Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of LPHN1 expression in rat kidney (A), Hela (B) whole cell lysates. (Predicted band size: 162 kD; Observed band size: 163 kD)
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Immunofluorescent analysis of LPHN1 staining in LOVO cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a AF594-conjugated secondary antibody (red) in PBS at room temperature in the dark.