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CPA3550
  • Western blot analysis of CD156a expression in mouse lung (A), mouse brain (B), rat spleen (C), rat lung (D), rat brain (E), rat spleen (F) whole cell lysates. (Predicted band size: 88 kD; Observed band size: 60 kD)
  • Immunofluorescent analysis of CD156a staining in SGC7901 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark. Phalloidin - AF594 was used to stain Actin filaments (red). DAPI was used to stain the cell nuclei (blue).
Product Name:Anti-CD156a Antibody
Cat No:CPA3550
Source:Rabbit
Reactivity:H, M, R
Applications:WB, IF/IC
*Application Key:
H - Human, M - Mouse, R - Rat, B - Bovine, C - Chicken, D - Dog, G - Goat, Mk - Monkey, P - Pig, Rb - Rabbit, S - Sheep, Z - Zebrafish
*Species Reactivity Key:
E- ELISA, WB - Western blot, IH - Immunohistochemistry, IF - Immunofluorescence, FC - Flow cytometry, IC - Immunocytochemistry, IP - Immunoprecipitation, ChIP - Chromatin Immunoprecipitation, EMSA - Electrophoretic Mobility Shift Assay, BL - Blocking, SE - Sandwich ELISA, CBE - Cell-based ELISA, RNAi - RNA interference
Size
Price(USD)
200 μl
350
100 μl
220
30 μl
110
50 μl
150
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Description:Rabbit polyclonal antibody to CD156a
Immunogen:KLH-conjugated synthetic peptide encompassing a sequence within the center region of human CD156a. The exact sequence is proprietary.
Purification:The antibody was purified by immunogen affinity chromatography.
Clonality:Polyclonal
Form:Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Dilution:WB (1/500 - 1/1000), IF/IC (1/50 - 1/200)
Gene Symbol:ADAM8
Alternative Names:MS2; Disintegrin and metalloproteinase domain-containing protein 8; ADAM 8; Cell surface antigen MS2; CD156a
Entrez Gene (Human): 101;
Entrez Gene (Mouse): 11501;
SwissProt (Human): P78325;
SwissProt (Mouse): Q05910;
Storage/Stability:Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
  • Western blot analysis of CD156a expression in mouse lung (A), mouse brain (B), rat spleen (C), rat lung (D), rat brain (E), rat spleen (F) whole cell lysates. (Predicted band size: 88 kD; Observed band size: 60 kD)
  • Immunofluorescent analysis of CD156a staining in SGC7901 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark. Phalloidin - AF594 was used to stain Actin filaments (red). DAPI was used to stain the cell nuclei (blue).
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