Description：Rabbit polyclonal antibody to ACTN1/2/3/4Immunogen：KLH-conjugated synthetic peptide encompassing a sequence within the N-term region of human ACTN1/2/3/4. The exact sequence is proprietary.Purification：The antibody was purified by immunogen affinity chromatography.Clonality：PolyclonalForm：Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.Dilution：WB (1/500 - 1/1000), IH (1/50 - 1/100)Gene Symbol：ACTN1; ACTN2; ACTN3; ACTN4Alternative Names：ACTN1; Alpha-actinin-1; Alpha-actinin cytoskeletal isoform; F-actin cross-linking protein; Non-muscle alpha-actinin-1; ACTN2; Alpha-actinin-2; Alpha-actinin skeletal muscle isoform 2; F-actin cross-linking protein; ACTN3; Alpha-actinin-3; Alpha-actinin skeletal muscle isoform 3; F-actin cross-linking protein; ACTN4; Alpha-actinin-4; F-actin cross-linking protein; Non-muscle alpha-actinin 4 Entrez Gene (Human)： 87; 88; 89; 81; Entrez Gene (Mouse)： 109711; 11472; 11474; 60595; Entrez Gene (Rat)： 81634; 63836; SwissProt (Human)： P12814; P35609; Q08043; O43707; SwissProt (Mouse)： Q7TPR4; Q9JI91; O88990; P57780; SwissProt (Rat)： Q9Z1P2; Q9QXQ0; Storage/Stability：Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

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  Western blot analysis of ACTN1/2/3/4 expression in A375 (A), U2OS (B), DLD (C), mouse heart (D), mouse muscle (E), rat heart (F), rat muscle (G) whole cell lysates. (Predicted band size: 103; 104 kD; Observed band size: 105 kD)
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  Immunohistochemical analysis of ACTN1/2/3/4 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.