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CPA3447
  • Western blot analysis of USP32 expression in A549 (A), MCF7 (B), PC12 (C), AML12 (D) whole cell lysates. (Predicted band size: 181 kD; Observed band size: 181 kD)
  • Immunohistochemical analysis of USP32 staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Product Name:Anti-USP32 Antibody
Cat No:CPA3447
Source:Rabbit
Reactivity:H, M, R, D, S
Applications:WB, IH
*Application Key:
H - Human, M - Mouse, R - Rat, B - Bovine, C - Chicken, D - Dog, G - Goat, Mk - Monkey, P - Pig, Rb - Rabbit, S - Sheep, Z - Zebrafish
*Species Reactivity Key:
E- ELISA, WB - Western blot, IH - Immunohistochemistry, IF - Immunofluorescence, FC - Flow cytometry, IC - Immunocytochemistry, IP - Immunoprecipitation, ChIP - Chromatin Immunoprecipitation, EMSA - Electrophoretic Mobility Shift Assay, BL - Blocking, SE - Sandwich ELISA, CBE - Cell-based ELISA, RNAi - RNA interference
Size
Price(USD)
200 μl
350
100 μl
220
30 μl
110
50 μl
150
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Description:Rabbit polyclonal antibody to USP32
Immunogen:KLH-conjugated synthetic peptide encompassing a sequence within the center region of human USP32. The exact sequence is proprietary.
Purification:The antibody was purified by immunogen affinity chromatography.
Clonality:Polyclonal
Form:Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Dilution:WB (1/500 - 1/1000), IH (1/100 - 1/200)
Gene Symbol:USP32
Alternative Names:USP10; Ubiquitin carboxyl-terminal hydrolase 32; Deubiquitinating enzyme 32; Renal carcinoma antigen NY-REN-60; Ubiquitin thioesterase 32; Ubiquitin-specific-processing protease 32
Entrez Gene (Human): 84669;
SwissProt (Human): Q8NFA0;
Storage/Stability:Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
  • Western blot analysis of USP32 expression in A549 (A), MCF7 (B), PC12 (C), AML12 (D) whole cell lysates. (Predicted band size: 181 kD; Observed band size: 181 kD)
  • Immunohistochemical analysis of USP32 staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
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