Description:Rabbit polyclonal antibody to EMPImmunogen:KLH-conjugated synthetic peptide encompassing a sequence within the center region of human EMP. The exact sequence is proprietary.Purification:The antibody was purified by immunogen affinity chromatography.Clonality:PolyclonalForm:Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.Dilution:WB (1/500 - 1/1000), IH (1/100 - 1/200)Gene Symbol:MAEAAlternative Names:EMP; Macrophage erythroblast attacher; Cell proliferation-inducing gene 5 protein; Erythroblast macrophage protein; Human lung cancer oncogene 10 protein; HLC-10
Entrez Gene (Human):
10296;
Entrez Gene (Mouse):
59003;
Entrez Gene (Rat):
298982;
SwissProt (Human):
Q7L5Y9;
SwissProt (Mouse):
Q4VC33;
SwissProt (Rat):
Q5RKJ1;
Storage/Stability:Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of EMP expression in HepG2 (A), HeLa (B) whole cell lysates. (Predicted band size: 45 kD; Observed band size: 45 kD)
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Immunohistochemical analysis of EMP staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Evaluation of erythroblast macrophage protein related to erythroblastic islands in patients with hematopoietic stem cell transplantation