Description：Rabbit polyclonal antibody to Separase (Phospho-S1126/801)Immunogen：KLH-conjugated synthetic phosphopeptide corresponding to residues surrounding S1126/801 of human Separase protein. The exact sequence is proprietary.Purification：The antibody was purified by immunogen affinity chromatography.Clonality：PolyclonalForm：Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.Dilution：WB (1/500 - 1/1000), IH (1/100 - 1/200), IF/IC (1/100 - 1/500)Gene Symbol：ESPL1Alternative Names：ESP1; KIAA0165; Separin; Caspase-like protein ESPL1; Extra spindle poles-like 1 protein; Separase Entrez Gene (Human)： 9700; SwissProt (Human)： Q14674; Storage/Stability：Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

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  Western blot analysis of Separase (Phospho-S1126/801) expression in MDA-MB-231 (A), A431 (B) whole cell lysates. (Predicted band size: 233 kD; Observed band size: 233 kD)
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  Immunohistochemical analysis of Separase (Phospho-S1126/801) staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
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  Immunofluorescent analysis of Separase (Phospho-S1126/801) staining in MDAMB231 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.