Description:Rabbit polyclonal antibody to PLK1 (Phospho-T210)Immunogen:KLH-conjugated synthetic phosphopeptide corresponding to residues surrounding T210 of human PLK1 protein. The exact sequence is proprietary.Purification:The antibody was purified by immunogen affinity chromatography.Clonality:PolyclonalForm:Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.Dilution:WB (1/500 - 1/1000), IH (1/100 - 1/200)Gene Symbol:PLK1Alternative Names:PLK; Serine/threonine-protein kinase PLK1; Polo-like kinase 1; PLK-1; Serine/threonine-protein kinase 13; STPK13
Entrez Gene (Human):
5347;
SwissProt (Human):
P53350;
Storage/Stability:Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of PLK1 (Phospho-T210) expression in HeLa (A), A549 (B), K562 (C), COLO320 (D) whole cell lysates. (Predicted band size: 68 kD; Observed band size: 68 kD)
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Immunohistochemical analysis of PLK1 (Phospho-T210) staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.