Description：Rabbit polyclonal antibody to AF4Immunogen：KLH-conjugated synthetic peptide encompassing a sequence within the N-term region of human AF4. The exact sequence is proprietary.Purification：The antibody was purified by immunogen affinity chromatography.Clonality：PolyclonalForm：Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.Dilution：WB (1/500 - 1/1000), IH (1/100 - 1/200)Gene Symbol：AFF1Alternative Names：AF4; FEL; MLLT2; PBM1; AF4/FMR2 family member 1; ALL1-fused gene from chromosome 4 protein; Protein AF-4; Protein FEL; Proto-oncogene AF4 Entrez Gene (Human)： 4299; SwissProt (Human)： P51825; Storage/Stability：Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

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  Western blot analysis of AF4 expression in HeLa (A), K562 (B), Jurkat (C) whole cell lysates. (Predicted band size: 131; 132 kD; Observed band size: 135 kD)
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  Immunohistochemical analysis of AF4 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.