Description：Rabbit polyclonal antibody to SMAD3 (Phospho-S425)Immunogen：KLH-conjugated synthetic phosphopeptide corresponding to residues surrounding S425 of human SMAD3 protein. The exact sequence is proprietary.Purification：The antibody was purified by immunogen affinity chromatography.Clonality：PolyclonalForm：Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.Dilution：WB (1/500 - 1/1000), IH (1/100 - 1/200)Gene Symbol：SMAD3Alternative Names：MADH3; Mothers against decapentaplegic homolog 3; MAD homolog 3; Mad3; Mothers against DPP homolog 3; hMAD-3; JV15-2; SMAD family member 3; SMAD 3; Smad3; hSMAD3 Entrez Gene (Human)： 4088; SwissProt (Human)： P84022; Storage/Stability：Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

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  Western blot analysis of SMAD3 (Phospho-S425) expression in A431 EGF-treated (A), HL60 TGF-treated (B) whole cell lysates. (Predicted band size: 48 kD; Observed band size: 60 kD)
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  Immunohistochemical analysis of SMAD3 (Phospho-S425) staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.