Description：Rabbit polyclonal antibody to Cortactin (Phospho-Y466)Immunogen：KLH-conjugated synthetic phosphopeptide corresponding to residues surrounding Y466 of human Cortactin protein. The exact sequence is proprietary.Purification：The antibody was purified by immunogen affinity chromatography.Clonality：PolyclonalForm：Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.Dilution：WB (1/500 - 1/1000), IH (1/50 - 1/100), IF/IC (1/50 - 1/200)Gene Symbol：CTTNAlternative Names：EMS1; Src substrate cortactin; Amplaxin; Oncogene EMS1 Entrez Gene (Human)： 2017; Entrez Gene (Mouse)： 13043; SwissProt (Human)： Q14247; SwissProt (Mouse)： Q60598; Storage/Stability：Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

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  Western blot analysis of Cortactin (Phospho-Y466) expression in HeLa UV-treated (A), NIH3T3 H2O2-treated (B) whole cell lysates. (Predicted band size: 61 kD; Observed band size: 85 kD)
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  Immunohistochemical analysis of Cortactin (Phospho-Y466) staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
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  Immunofluorescent analysis of Cortactin (Phospho-Y466) staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).