Description：Rabbit polyclonal antibody to Annexin A2 (Phospho-S26)Immunogen：KLH-conjugated synthetic phosphopeptide corresponding to residues surrounding S26 of human Annexin A2 protein. The exact sequence is proprietary.Purification：The antibody was purified by immunogen affinity chromatography.Clonality：PolyclonalForm：Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.Dilution：WB (1/500 - 1/1000), IF/IC (1/50 - 1/200)Gene Symbol：ANXA2Alternative Names：ANX2; ANX2L4; CAL1H; LPC2D; Annexin A2; Annexin II; Annexin-2; Calpactin I heavy chain; Calpactin-1 heavy chain; Chromobindin-8; Lipocortin II; Placental anticoagulant protein IV; PAP-IV; Protein I; p36 Entrez Gene (Human)： 302; Entrez Gene (Mouse)： 12306; Entrez Gene (Rat)： 56611; SwissProt (Human)： P07355; SwissProt (Mouse)： P07356; SwissProt (Rat)： Q07936; Storage/Stability：Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

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  Western blot analysis of Annexin A2 (Phospho-S26) expression in mouse lung (A), rat lung (B), rat liver (C) whole cell lysates. (Predicted band size: 38 kD; Observed band size: 38 kD)
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  Immunofluorescent analysis of Annexin A2 (Phospho-S26) staining in A549 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark. Phalloidin - AF594 was used to stain Actin filaments (red). DAPI was used to stain the cell nuclei (blue).