Description：Rabbit polyclonal antibody to Androgen Receptor (Phospho-S650)Immunogen：KLH-conjugated synthetic phosphopeptide corresponding to residues surrounding S650 of human Androgen Receptor protein. The exact sequence is proprietary.Purification：The antibody was purified by immunogen affinity chromatography.Clonality：PolyclonalForm：Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.Dilution：WB (1/500 - 1/1000), IH (1/100 - 1/500)Gene Symbol：ARAlternative Names：DHTR; NR3C4; Androgen receptor; Dihydrotestosterone receptor; Nuclear receptor subfamily 3 group C member 4 Entrez Gene (Human)： 367; Entrez Gene (Mouse)： 11835; Entrez Gene (Rat)： 24208; SwissProt (Human)： P10275; SwissProt (Mouse)： P19091; SwissProt (Rat)： P15207; Storage/Stability：Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

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  Western blot analysis of Androgen Receptor (Phospho-S650) expression in H1688 (A), mouse liver (B), mouse kidney (C), rat liver (D), rat kidney (E) whole cell lysates. (Predicted band size: 99; 110 kD; Observed band size: 120 kD)
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  Immunohistochemical analysis of Androgen Receptor (Phospho-S650) staining in human prostate cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
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  Direct ELISA antibody dose-response curve using Anti-Androgen Receptor (Phospho-S650) Antibody. Antigen (Phosphopeptide and non-phosphopeptide) concentration is 5 ug/ml. Goat Anti-Rabbit IgG (H&L) - HRP was used as the secondary antibody, and signal was developed by TMB substrate.