Description：Rabbit polyclonal antibody to Cytochrome P450 8B1Immunogen：KLH-conjugated synthetic peptide encompassing a sequence within the center region of human Cytochrome P450 8B1. The exact sequence is proprietary.Purification：The antibody was purified by immunogen affinity chromatography.Clonality：PolyclonalForm：Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.Dilution：WB (1/500 - 1/1000), IF/IC (1/50 - 1/200)Gene Symbol：CYP8B1Alternative Names：CYP12; 7-alpha-hydroxycholest-4-en-3-one 12-alpha-hydroxylase; 7-alpha-hydroxy-4-cholesten-3-one 12-alpha-hydroxylase; CYPVIIIB1; Cytochrome P450 8B1; Sterol 12-alpha-hydroxylase Entrez Gene (Human)： 1582; Entrez Gene (Mouse)： 13124; SwissProt (Human)： Q9UNU6; SwissProt (Mouse)： O88962; Storage/Stability：Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

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  Western blot analysis of Cytochrome P450 8B1 expression in 3T3L1 (A), A375 (B), rat liver (C), LO2 (D), HepG2 (E) whole cell lysates. (Predicted band size: 58 kD; Observed band size: 58 kD)
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  Immunofluorescent analysis of Cytochrome P450 8B1 staining in MCF7 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark. Phalloidin - AF594 was used to stain Actin filaments (red). DAPI was used to stain the cell nuclei (blue).