Description：Rabbit polyclonal antibody to COX8AImmunogen：KLH-conjugated synthetic peptide encompassing a sequence within the N-term region of human COX8A. The exact sequence is proprietary.Purification：The antibody was purified by immunogen affinity chromatography.Clonality：PolyclonalForm：Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.Dilution：WB (1/500 - 1/1000), IF/IC (1/50 - 1/200)Gene Symbol：COX8AAlternative Names：COX8; COX8L; Cytochrome c oxidase subunit 8A mitochondrial; Cytochrome c oxidase polypeptide VIII-liver/heart; Cytochrome c oxidase subunit 8-2 Entrez Gene (Human)： 1351; Entrez Gene (Mouse)： 12868; Entrez Gene (Rat)： 171335; SwissProt (Human)： P10176; SwissProt (Mouse)： Q64445; SwissProt (Rat)： P80433; Storage/Stability：Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

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  Western blot analysis of COX8A expression in A549 (A), SP2/0 (B), rat brain (C) whole cell lysates. (Predicted band size: 7 kD; Observed band size: 7 kD)
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  Immunofluorescent analysis of COX8A staining in HCCLM3 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark. Phalloidin - AF594 was used to stain Actin filaments (red). DAPI was used to stain the cell nuclei (blue).