Description:Rabbit polyclonal antibody to EOSImmunogen:KLH-conjugated synthetic peptide encompassing a sequence within the center region of human EOS. The exact sequence is proprietary.Purification:The antibody was purified by immunogen affinity chromatography.Clonality:PolyclonalForm:Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.Dilution:WB (1/500 - 1/1000), IH (1/50 - 1/100)Gene Symbol:PRSS33Alternative Names:Serine protease 33; Serine protease EOS
Entrez Gene (Human):
260429;
Entrez Gene (Mouse):
353130;
SwissProt (Human):
Q8NF86;
SwissProt (Mouse):
Q80WM7;
Storage/Stability:Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of EOS expression in Jurkat (A), HGC27 (B), mouse brain (C), rat spleen (D), rat brain (E) whole cell lysates. (Predicted band size: 29 kD; Observed band size: 33 kD)
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Immunohistochemical analysis of EOS staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.