Description:Rabbit polyclonal antibody to Calpain 12Immunogen:KLH-conjugated synthetic peptide encompassing a sequence within the center region of human Calpain 12. The exact sequence is proprietary.Purification:The antibody was purified by immunogen affinity chromatography.Clonality:PolyclonalForm:Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.Dilution:WB (1/500 - 1/1000), IH (1/50 - 1/100)Gene Symbol:CAPN12Alternative Names:Calpain-12; Calcium-activated neutral proteinase 12; CANP 12
Entrez Gene (Human):
147968;
SwissProt (Human):
Q6ZSI9;
Storage/Stability:Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of Calpain 12 expression in HepG2 (A), A549 (B) whole cell lysates. (Predicted band size: 81 kD; Observed band size: 81 kD)
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Immunohistochemical analysis of Calpain 12 staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.