Description:Rabbit polyclonal antibody to FGFR1 (Phospho-Y766)Immunogen:KLH-conjugated synthetic phosphopeptide corresponding to residues surrounding Y766 of human FGFR1 protein. The exact sequence is proprietary.Purification:The antibody was purified by immunogen affinity chromatography.Clonality:PolyclonalForm:Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.Dilution:WB (1/500 - 1/1000), IH (1/100 - 1/200)Gene Symbol:FGFR1Alternative Names:BFGFR; CEK; FGFBR; FLG; FLT2; HBGFR; Fibroblast growth factor receptor 1; FGFR-1; Basic fibroblast growth factor receptor 1; BFGFR; bFGF-R-1; Fms-like tyrosine kinase 2; FLT-2; N-sam; Proto-oncogene c-Fgr; CD331
Entrez Gene (Human):
2260;
Entrez Gene (Mouse):
14182;
Entrez Gene (Rat):
79114;
SwissProt (Human):
P11362;
SwissProt (Mouse):
P16092;
SwissProt (Rat):
Q04589;
Storage/Stability:Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of FGFR1 (Phospho-Y766) expression in HepG2 EGF-treated (A), SP2/0 EGF-treated (B), PC12 EGF-treated (C) whole cell lysates. (Predicted band size: 91 kD; Observed band size: 145 kD)
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Immunohistochemical analysis of FGFR1 (Phospho-Y766) staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Fibronectin induces endothelial cell migration through ß1 integrin and Src-dependent phosphorylation of fibroblast growth factor receptor-1 at tyrosines 653/654 and 766